Preparing a microscope slide.

 

slide

Rectangular piece of glass on which you place the specimen.

   

coverslip

Thin, square piece of glass which you place over the specimen on the slide.

 

How to prepare a Wet Mount
A wet mount requires water (slide, water, object, coverslip. It is used to prepare slides that hold living organisms.

 

1. Place slide on a flat surface.
2. Place a drop of water on the slide. Add the specimen to the drop of water (at times, you may want to have the specimen already on the slide before adding the water).
3. Hold the coverslip by its sides and lay its bottom edge on the slide close to the specimen. Holding the coverslip at a 45 angle helps.
4. Slowly lower the coverslip so that it spreads the water out. If you get air bubbles (looking like little black doughnuts), gently press on the coverslip to move them to the edge. If there are dry areas under the coverslip, add a little more water at the edge of the coverslip. Too much water can be dabbed off with a piece of paper towel.
 

 

 

 

 

 

 

Staining specimens - iodine or methylene blue may be added to specimens in order to increase contrast. The stain can be directly added to the water when first preparing the slide or it can be added later after first viewing the specimen without the stain. Add a drop of the stain along one edge of the coverslip. Placing a piece of paper towel along the opposite edge of the coverslip will help draw the stain under the coverslip.